PASAP Hydrogel

PASAP Hydrogel

PASAP (Pro-Adhesion Self-Assembling Peptide) Hydrogel is a synthetic matrix used to create defined three-dimensional (3D) micro-environments for a variety of cell culture experiments. PASAP nanostructured hydrophilic hydrogel displays multiple functionalizations that foster cell attachment and spreading, providing a nanostructured synthetic microenvironment that can be tuned to specific cell types fostering differentiation of a variety of cells such as Neural Stem Cells, Colon stem cells, endothelial cells and fibroblasts.

 

PASAP provides the typical nanostructured topography of the extracellular matrix, thus mimicking the physiological microenvironment surrounding cells found in living tissues. This nanostructure supports cell attachment and spreading, thus fostering branching and differentiation: a paramount result of great importance to future therapeutic applications.

PASAP Hydrogel consists of a well-defined mixture of standard synthetic amino acids (1% w/v) and 99% water. Under physiological conditions, the peptide component self-assembles into a 3D hydrogel that exhibits a nano-fibrous structure. The hydrogel can be readily formed in culture dishes, multi-well plates, or cell culture inserts. ASSAP applications include 2D and 3D cell cultures and in vivo cellular or drug therapies.

PASAP Hydrogel has been shown to promote survival (figure 1) and differentiation (figure 2) of murine and human Neural Stem Cells, of human Colon Stem Cells, of murine endothelial cells and of human fibroblasts. Preliminarystudies also show that PASAP Hydrogel supports the in vivo engraftment of transplanted Neural Stem Cells into the site of acute Spinal Cord Injuries in Rats. Other potential applications include generic stem cell differentiation, tumor cell migration and invasion, angiogenesis assays, and in vivo therapies of tissue regeneration

FIGURE 1

Murine Neural Stem Cells (left) and Human Neural Stem Cells (right) on PASAP Peptide Hydrogel in 2D cell culture conditions (7 DIV). PASAP favors cell adhesion thus triggering stem cell differentiation. Live Cells are marked in green. NSC cultured on untreated plastic wells do not attach and stay in round-shaped clusters. 5x magnification.

FIGURE 2

Differentiated murine Neural Stem Cells on PASAP Peptide Hydrogel (96 MW) in differentiating cell culture medium (7 DIV). Cultures are serum-free and no animal-component has been used for tailoring the scaffold. Astrocytes and neurons are marked respectively in red (GFAP) and in green (bTubulin). PASAP fosters cell adhesion thus stem cell differentiation in networks of differentiating cell progenies. Cells are cultured in a three-dimensional fashion. 20x magnification.

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