Anti-Spreading Self Assembling Peptide or ASSAP
ASSAP Hydrogel is a synthetic matrix that is used to create defined three-dimensional (3D) micro-environments for a variety of cell culture experiments. ASSAP nanostructured hydrophilic hydrogel inhibits cell attachment and spreading and provides a nanostructured synthetic microenvironment that can be tuned to specific cell types allowing the culture of “difficult-to-culture” cells such as Lung, Breast and Colon stem cells.
The first Nanomed3D SAP available for distribution. ASSAP provides the typical nanostructured topography of the extracellular matrix, thus mimicking the physiological niches of stem cells found in tissues.
This minimizes cell spreading without preventing cell low-attachment, thus fostering their proliferation and preserving their stemness: a paramount result of great importance to future therapeutic applications.
ASSAP Hydrogel consists of a well-defined mixture of standard synthetic amino acids (1% w/v) and 99% water. Under physiological conditions, the peptide component self-assembles into a 3D hydrogel that exhibits a nanometer scale fibrous structure (Figure 1).
The hydrogel can be readily formed in culture dishes, multi-well plates, or cell culture inserts. ASSAP applications include 2D and 3D cell cultures and in vivo cellular or drug therapies.
Figure 1
The first Nanomed3D SAP available for distribution. ASSAP provides the typical nanostructured topography of the extracellular matrix, thus mimicking the physiological niches of stem cells found in tissues.
This minimizes cell spreading without preventing cell low-attachment, thus fostering their proliferation and preserving their stemness: a paramount result of great importance to future therapeutic applications.
ASSAP Hydrogel consists of a well-defined mixture of standard synthetic amino acids (1% w/v) and 99% water. Under physiological conditions, the peptide component self-assembles into a 3D hydrogel that exhibits a nanometer scale fibrous structure (Figure 1).
Figure 1
The hydrogel can be readily formed in culture dishes, multi-well plates, or cell culture inserts. ASSAP applications include 2D and 3D cell cultures and in vivo cellular or drug therapies.
Figure 2
Figure 2. Murine Neural Stem Cells in ASSAP Peptide Hydrogel (left) and on untreated plastic (right) bottom well (96 MW) in proliferating cell culture medium (7 DIV). ASSAP prevents cell adhesion thus preserving stem cell proliferation in neurospheres. Cells are cultured in a three-dimensional fashion.
Technical specifications
1% solution (w/v) of purified synthetic peptides
Packaged material exhibits pH = 3-5
Quality
Cell viability ≥ 80% based on cytotoxicity analysis of Neural Stem Cells.
Purity ≥ 99% confirmed using HPLC, LC-MS and MALDI-TOF.
Demonstration of fiber formation using self-assembly and rheology assays.
Features
Purified Synthetic Peptide Composition
Highly defined material that prevents cell spreading
3D Hydrogel Structure
Assembles into fibrous structure with average pore size of 50-200 nm
Easy Handling
Easily mixed with cells and/or bioactive molecules (e.g., growth factors) prior to gelation; injectable for in vivo studies
Transparent Hydrogel
Samples are readily visualized using standard staining methodologies and microscopy.
Established Protocols
3D cell encapsulation cultures; surface plating of cells on microporous membrane inserts and multi-wells; cell recovery for sub-culturing or biochemical analyses; in vivo injection.
Features
Purified Synthetic Peptide Composition
Highly defined material that prevents cell spreading
3D Hydrogel Structure
Assembles into fibrous structure with average pore size of 50-200 nm
Easy Handling
Easily mixed with cells and/or bioactive molecules (e.g., growth factors) prior to gelation; injectable for in vivo studies
Transparent Hydrogel
Samples are readily visualized using standard staining methodologies and microscopy.
Established Protocols
3D cell encapsulation cultures; surface plating of cells on microporous membrane inserts and multi-wells; cell recovery for sub-culturing or biochemical analyses; in vivo injection.
Usage
Peptide sequence prevents cell spreading but favors attachment: does not activate RGD-dependent integrin signaling.
In the presence of salt-containing solution, the peptide component of ASSAP Hydrogel self-assembles and forms a transparent 3D hydrogel.
Biocompatible; devoid of animal-derived material and pathogens.
Application
2 dimensional and 3 dimensional cell cultures of Neural Stem Cells, Breast Stem Cells, Colon Stem Cells, Lung Stem Cells.
Injectable scaffold and carrier of stem cells for regenerative medicine applications.
FOR RESEARCH USE ONLY, NOT FOR CLINICAL OR DIAGNOSTIC USAGE.
Product Details
Name
Size
Solvent
Regulatory Status
Storage
ASSAP Hydrogel
5 mL
dH2O
RUO
+2 – +6° C
ASSAP Hydrogel
10 mL
dH2O
RUO
+2 – +6° C
Product Details
Name
ASSAP Hydrogel
Size
5 mL, 10mL
Solvent
dH2O
Regulatory Status
RUO
Storage
+2 – +6° C